A reagent for improved purity and yield of biofluid-derived extracellular vesicles (EVs)

A reagent for improved purity and yield of biofluid-derived extracellular vesicles (EVs)

Key features:

• Simple and effective method for improved removal of lipoproteins, major contaminants in biofluid-derived EVs.
• Improved EV yield while preserving EV morphology.
• Compatible with industry standard purification methods including TFF and SEC

Background

Extracellular vesicles (EVs) are nanosized biomolecular packages involved in intercellular communication. EVs are released by all cell types, making them broadly applicable in therapeutic and diagnostic applications. EVs are a promising next generation cell-free therapy and drug delivery platform and have potential as biomarkers for diseases diagnosis.

Sample purity is critical to maximise performance and correctly attribute observed therapeutic or diagnostic effects to EVs rather than other biological particles. Lipoprotein contaminants represent a major challenge for obtaining pure samples of biofluid-derived EVs.