The ability to amplify and detect specific DNA sequences is a powerful tool routinely used for a wide variety of applications, including disease diagnostics and mutant screening. With the development of isothermal DNA amplification methods, such as loop-mediated isothermal amplification (LAMP), the need for expensive laboratory thermocyclers for molecular-based diagnostics has now been eliminated. However, the requirement for low-lost, simple and equipment-free DNA readout methods remains.

Researchers at the University of Queensland have developed a rapid method to visualise amplified DNA in just one-step, without requiring any equipment. The readout system is made possible by a unique combination of suspended nanoparticles within a special DNA-detection solution.

Key features

  • Detects amplified DNA in 10-30 seconds
  • Equipment-free – results read by the naked eye
  • Amplification and detection performed in a single tube
  • Ideal for point-of-need diagnostics.

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