Knock-in genetic barcodes for multiplexed single-cell sequencing

To overcome the high cost of processing single-cell RNA sequencing, multiplexing methods have emerged that accelerate knowledge gain in gene and drug discovery.  Development of genetically encoded barcodes in isogenic cell lines overcome key limitations with existing barcoding methods.

Researchers at The University of Queensland (UQ) have generated a method for barcoding human cells with proprietary barcodes that enables scalable multiplexed single cell RNA sequencing.  The UQ technology platform provides trillions of barcoding options for engineering any human cell line, enabling a significantly expanded portfolio of experimental design options not possible with external barcoding methods.